D-(+)-Cellobiose (CNP0226604.15) → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Participants

Reaction Steps

To an enzyme liquid, cellobiose (Wako Pure Chemical Industries, Ltd.) was added at 500 mg/L and sodium acetate buffer (pH 5.0) was added at 100 mM, followed by allowing the resulting mixture to react at 50° C. for 0.5 hour. This reaction liquid was prepared in a 1-mL tube, and the reaction was allowed to proceed with mixing by rotation under the above-described conditions. Thereafter, the tube was subjected to centrifugation, and the glucose concentration in the supernatant component was measured. The measurement of glucose concentration was carried out according to the method described in Reference Example 2. The concentration of glucose produced (g/L) was used as it is as the activity level of the cellobiose-degrading activity, and used for comparison of the amount of enzyme recovered.

Provenance & Evidence