D-(+)-Cellobiose (CNP0226604.15) → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 1504352Notes

Reaction equation

HRID 1504352 reaction equation
[CH2:1]([OH:23])[C@H:2]1[O:7][C@@H:6]([O:8][C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22].C([O-])(=O)C.[Na+]>>[O:8]=[CH:6][C@@H:5]([C@H:4]([C@@H:3]([C@@H:2]([CH2:1][OH:23])[OH:7])[OH:22])[OH:21])[OH:20]

Participants

3

Reactant 2

HCID 10712D-(+)-Cellobiose (CNP0226604.15)HCID 517045Sodium Acetate

Product 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Procedure

To an enzyme liquid, cellobiose (Wako Pure Chemical Industries, Ltd.) was added at 500 mg/L and sodium acetate buffer (pH 5.0) was added at 100 mM, followed by allowing the resulting mixture to react at 50° C. for 0.5 hour. This reaction liquid was prepared in a 1-mL tube, and the reaction was allowed to proceed with mixing by rotation under the above-described conditions. Thereafter, the tube was subjected to centrifugation, and the glucose concentration in the supernatant component was measured. The measurement of glucose concentration was carried out according to the method described in Reference Example 2. The concentration of glucose produced (g/L) was used as it is as the activity level of the cellobiose-degrading activity, and used for comparison of the amount of enzyme recovered.

Workup

  1. additionwas added at 100 mM
  2. customto react at 50° C. for 0.5 hour
  3. customThis reaction liquid
  4. customwas prepared in a 1-mL tube
  5. additionwith mixing by rotation under the above-described conditions
  6. concentrationthe glucose concentration in the supernatant component
  7. concentrationThe measurement of glucose concentration

Sources & evidence

ORD record
ord-4b1250b95f344ab2ab2a1e67102c1c2b
Source dataset
uspto-grants-2015_12
Patent number
US09212377B2

Reaction notes

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