51110-01-1 → 38916-34-6

Participants

Reaction Steps

A pharmaceutical composition of somatostatin was prepared in 0.9% sodium chloride containing 20 mg of phosphatidyl choline and 2.7 mg of somatostatin-14 per mL. Briefly, phosphatidyl choline was slowly added under stirring to the 0.9% sodium chloride aqueous solution containing somatostatin-14. The composition was further stirred for 15 minutes until a liposome suspension was formed. Then liposomes were stepwise extruded through a polycarbonate filter with a 400 nm pore size, a 200 nm pore size filter and eventually ten times with a 100 nm pore size filter. Finally, the liposomal solution was filtered through a 0.22 μm sterilizing filter.

Provenance & Evidence