Ethanolamine → Oleoylethanolamide

Participants

Reaction Steps

In an exemplary embodiment, 1 mmol oleic acid was reacted with 1 mmol ethanolamine in 1.5 mL hexane at 65° C. for 3 hours in the presence 30% lipase and 10 μL water. N-oleoyl ethanolamine with 96.6% purity was obtained and all oleic acid was consumed. In another exemplary embodiment, when the synthesis was conducted on a large scale (50 mmol of each of the reactants), the purity of N-oleoyl ethanolamine after crystallization purification was 96.1%. More examples of reacting the alkanolamine and the fatty acid to prepare the high-purity fatty acid N-acylalkanolamine in a high yield are described in Examples 9-18.

Provenance & Evidence