Tromethamine hydrochloride → Deoxyguanosine

Participants

Reaction Steps

The washed cells described above were added to 50 mM Tris-HCl buffer (pH 7.2) containing 20.0 g/l 2,6-diaminopurine-2′-deoxyriboside to a concentration of 5 g/l and reacted at 30° C. for 3 hours while keeping pH at 7.2. The concentration of 2′-deoxyguanosine produced in the reaction solution was measured by high performance liquid chromatography. The result showed that the concentration was 19.8 g/l (conversion molar yield: 99%), respectively.

Provenance & Evidence