Tromethamine hydrochloride → Deoxyguanosine

HRID 1530779Notes

Reaction equation

HRID 1530779 reaction equation
C(O)C(N)(CO)C[OH:4].Cl.[CH2:10]1[C@H:14]([N:15]2[C:19]3[N:20]=[C:21]([NH2:25])[N:22]=[C:23](N)[C:18]=3[N:17]=[CH:16]2)[O:13][C@H:12]([CH2:26][OH:27])[C@H:11]1[OH:28]>>[C@@H:14]1([N:15]2[C:19]3[N:20]=[C:21]([NH2:25])[NH:22][C:23](=[O:4])[C:18]=3[N:17]=[CH:16]2)[O:13][C@H:12]([CH2:26][OH:27])[C@@H:11]([OH:28])[CH2:10]1

Participants

3

Reactant 2

HCID 93573Tromethamine hydrochlorideHCID 971884546-70-7

Product 1

HCID 135398592DeoxyguanosineYield 99%

Procedure

The washed cells described above were added to 50 mM Tris-HCl buffer (pH 7.2) containing 20.0 g/l 2,6-diaminopurine-2′-deoxyriboside to a concentration of 5 g/l and reacted at 30° C. for 3 hours while keeping pH at 7.2. The concentration of 2′-deoxyguanosine produced in the reaction solution was measured by high performance liquid chromatography. The result showed that the concentration was 19.8 g/l (conversion molar yield: 99%), respectively.

Workup

  1. customreacted at 30° C. for 3 hours

Sources & evidence

ORD record
ord-14735abe79464dbfa902eaebe22ab97d
Source dataset
uspto-grants-2001_03
Patent number
US06197552B1

Reaction notes

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