Sodium Bicarbonate → 5-(2-(4-Hydroxyphenyl)Ethenyl)Benzene-1,3-Diol

HRID 1531314Notes

Reaction equation

HRID 1531314 reaction equation
C([O-])(O)=O.[Na+].C[C@@H](N)[C@H]1O[C@H]([O:14][C@H:15]2[C@H:20](O)[C@@H:19]([O:22][C@H]3OC[C@@](O)(C)[C@H](NC)[C@H]3O)[C@H:18](N)[CH2:17][C@@H:16]2N)[C@H](N)CC1.C[C@@H](NC)[C@H]1O[C@H](O[C@H:47]2[C@H:52](O)[C@@H:51]([O:54][C@H]3OC[C@@](O)(C)[C@H](NC)[C@H]3O)[C@H:50](N)[CH2:49][C@@H:48]2N)[C@H](N)CC1.[CH3:71][C@@:72]1(O)[C@H](NC)[C@@H](O)[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@H]3O[C@H](CN)CC[C@H]3N)[C@@H](N)C[C@H]2N)OC1.OS(O)(=O)=O>>[C:17]1([CH:71]=[CH:72][C:48]2[CH:47]=[CH:52][C:51]([OH:54])=[CH:50][CH:49]=2)[CH:16]=[C:15]([OH:14])[CH:20]=[C:19]([OH:22])[CH:18]=1

Participants

3

Reactant 2

HCID 516892Sodium BicarbonateHCID 6419933Gentamicine sulfate

Product 1

HCID 50565-(2-(4-Hydroxyphenyl)Ethenyl)Benzene-1,3-Diol

Procedure

Cultures of African green monkey kidney cells (Vero) cells, obtained from the American Type Culture Collection, Rockville, Md., were grown to confluence in Medium 199 supplemented with 5% fetal bovine serum, 0.075% NaHCO3, and 50 μg/ml gentamycin sulfate in 25 cm2 tissue culture flasks. Cells were infected with HSV-1 at a multiplicity of infection (moi) of one and incubated at room temperature for one hour to allow for virus attachment to and penetration of the cell. Under these conditions, approximately half of the cells are infected with virus. Thereafter, the cultures were rinsed three time with media and incubated in medium containing resveratrol at a final concentration of 10 μg/ml, 25 μg/ml, or 50 μg/ml. Stock solutions of the resveratrol, obtained from Sigma Chemical Co, St. Louis, Mo. were prepared in 100% ethanol and diluted to the final concentration in tissue culture media. The concentration of alcohol in the medium ranged from 0.1 to 0.5%. Controls were treated identically, but were incubated without resveratrol.

Workup

  1. customCultures of African green monkey kidney cells (Vero) cells, obtained from the American Type Culture Collection, Rockville, Md
  2. washThereafter, the cultures were rinsed three time with media
  3. additioncontaining resveratrol at a final concentration of 10 μg/ml, 25 μg/ml, or 50 μg/ml
  4. customwere prepared in 100% ethanol
  5. additiondiluted to the final
  6. concentrationconcentration in tissue culture media
  7. additionControls were treated identically

Sources & evidence

ORD record
ord-44b84f6366c24c4bbcf3133e0d4b8e4c
Source dataset
uspto-grants-2001_03
Patent number
US06197834B1

Reaction notes

Loading…