反应详情

To a solution of 1-[3-{2-[benzyl-(4-pyridin-3-yl-butyl)-amino]ethyl}-2-(3,5-dimethylphenyl)-1H-indol-5-yl]-1,4-dihydrotetrazol-5-one (25 mg in 1.5 mL dry N,N-dimethylformamide) at 0° C. was added 13 mg potassium carbonate followed by 0.033 mL of a 10% solution of iodomethane in methylene cloride, and the mixture stirred at low temperature. After 2 hours, the reaction was quenched by the addition of saturated aqueous ammonium chloride and the mixture extracted with ethyl acetate. The organic portion was washed successively with water and brine, dried over sodium sulfate and concentrated in vacuo. Purification of the concentrate by flash chromatography on silica gel (methylene chloride:methanol, 95:5) gave the title compound (20 mg).

1. customthe reaction was quenched by the addition of saturated aqueous ammonium chloride
2. extractionthe mixture extracted with ethyl acetate
3. washThe organic portion was washed successively with water and brine
4. dry with materialdried over sodium sulfate
5. concentrationconcentrated in vacuo
6. customPurification of the
7. concentrationconcentrate by flash chromatography on silica gel (methylene chloride:methanol, 95:5)