HRID1534317

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：OC[C@H]1O[C@H](OC[C@H]2O[C@H](O[C@]3(CO)O[C@H](CO)[C@@H](O)[C@@H]3O)[C@H](O)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@H]1O

HCID439242

Raffinose

C18H32O16

2 次

$结构：CCCCCCCC/C=C\CCCCCCCC(=O)O[C@H](COC(=O)CCCCCCCCCCCCCCC)COP(=O)([O-])OCC[N+](C)(C)C.N[C@H](C(=O)O)[C@H](O)c1ccc(O)c(O)c1$

HCID131717168

未命名化合物

PRODUCTS

生成物

结构：OC[C@H]1O[C@@](CO)(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H]1O

HCID5988

Sucrose

C12H22O11

收率 10%

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID1061

Phosphate

O4P-3

PROCEDURE

实验过程

A sonicated POPC/DOPS dispersion in phosphate buffer plus 10% sucrose (mole ratio 7:3) is prepared as described previously. 3H --labeled raffinose is entrapped in the vesicle cavity and external [3H,]- raffinose is removed by gel filtration on Sephadex G-50 as described previously. The lipid dispersion consisting of SUV with entrapped raffinose was chromatographed on Sepharose 4B before and after spray-drying. Before spray-drying it was found that 95% of the raffinose eluted together with the vesicle peak. After spray-drying 85% of the raffinose was eluted together with SUV. This experiment thus demonstrates that raffinose remains essentially entrapped during spray-drying at least to 90%.

WORKUP

后处理

customis removed by gel filtration on Sephadex G-50
customwas chromatographed on Sepharose 4B before and
customafter spray-drying
customBefore spray-drying it
washeluted together with the vesicle peak
dry with materialAfter spray-drying 85% of the raffinose
washwas eluted together with SUV
customduring spray-drying at least to 90%