Raffinose → Sucrose

HRID 1534317Notes

Reaction equation

HRID 1534317 reaction equation
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

Participants

4

Reactant 2

HCID 439242Raffinose2×HCID 131717168HCID 131717168, compound

Product 1

HCID 5988SucroseYield 10%

Reagents, catalysts & solvents 1

HCID 1061PhosphateSolvent

Procedure

A sonicated POPC/DOPS dispersion in phosphate buffer plus 10% sucrose (mole ratio 7:3) is prepared as described previously. 3H --labeled raffinose is entrapped in the vesicle cavity and external [3H,]- raffinose is removed by gel filtration on Sephadex G-50 as described previously. The lipid dispersion consisting of SUV with entrapped raffinose was chromatographed on Sepharose 4B before and after spray-drying. Before spray-drying it was found that 95% of the raffinose eluted together with the vesicle peak. After spray-drying 85% of the raffinose was eluted together with SUV. This experiment thus demonstrates that raffinose remains essentially entrapped during spray-drying at least to 90%.

Workup

  1. customis removed by gel filtration on Sephadex G-50
  2. customwas chromatographed on Sepharose 4B before and
  3. customafter spray-drying
  4. customBefore spray-drying it
  5. washeluted together with the vesicle peak
  6. dry with materialAfter spray-drying 85% of the raffinose
  7. washwas eluted together with SUV
  8. customduring spray-drying at least to 90%

Sources & evidence

ORD record
ord-dd95e8fa10884edba9918f15a1442b6b
Source dataset
uspto-grants-1992_02
Patent number
US05089181

Reaction notes

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