Phosphate → Morphine

Participants

Reaction Steps

The reagents which were employed were as follows: FLUMO (fluorescein-morphine conjugate) was 3 × 10-6M in water; antifluorescein, was 5.6 × 10-6M in binding sites, in water, 0.05M phosphate, pH 8.0; antimorphine was 2 × 10-4M in binding sites, 0.05M Tris-HCl, pH 8.0, in saline solution; buffer was Tris/saline 0.05M/1, pH 8.0. Opiate solutions had 1,000μg/ml per ml of codeine.

Provenance & Evidence