NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

HRID 1582955Notes

Reaction equation

HRID 1582955 reaction equation
[CH:1]1[CH:6]=[N+:5]([C@@H:7]2[O:11][C@H:10]([CH2:12][O:13][P:14]([O:17][P:18]([O:21][CH2:22][C@H:23]3[O:27][C@@H:26]([N:28]4[C:32]5[N:33]=[CH:34][N:35]=[C:36]([NH2:37])[C:31]=5[N:30]=[CH:29]4)[C@H:25]([O:38][P:39]([OH:42])([OH:41])=[O:40])[C@@H:24]3[OH:43])([OH:20])=[O:19])([OH:16])=[O:15])[C@@H:9]([OH:44])[C@H:8]2[OH:45])[CH:4]=[C:3]([C:46]([NH2:48])=[O:47])[CH:2]=1.C(OP(O)(O)=O)[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O.[Mg+2].[Cl-].[Cl-]>>[CH:34]1[N:35]=[C:36]([NH2:37])[C:31]2[N:30]=[CH:29][N:28]([C@@H:26]3[O:27][C@H:23]([CH2:22][O:21][P:18]([O:17][P:14]([O:13][CH2:12][C@H:10]4[O:11][C@@H:7]([N:5]5[CH:4]=[C:3]([C:46]([NH2:48])=[O:47])[CH2:2][CH:1]=[CH:6]5)[C@H:8]([OH:45])[C@@H:9]4[OH:44])([OH:16])=[O:15])([OH:20])=[O:19])[C@@H:24]([OH:43])[C@H:25]3[O:38][P:39]([OH:42])([OH:41])=[O:40])[C:32]=2[N:33]=1

Participants

4

Reactant 3

HCID 5886NADP nicotinamide-adenine-dinucleotide phosphateHCID 5958D-Glucopyranose 6-(dihydrogen phosphate)2×HCID 5360315Magnesium Chloride

Product 1

HCID 5884NADPH

Conditions

Temperature
37 °C

Procedure

An NADPH production system was prepared by mixing 42 mg of NADP, 5 ml of 100 mM glucose-6-phosphatase (G6P) and 5 ml of 100 mM MgCl2, and adding 57 μl of G6P dehydrogenase (about 1750 U/5 mg/ml) to the mixture. This was heated at 37° C. for 5 minutes and then ice-cooled until its use.

Workup

  1. temperatureice-cooled until its use

Sources & evidence

ORD record
ord-2b1729cba9ad4a8ba3d1924150447f8e
Source dataset
uspto-grants-2004_05
Patent number
US06740662B1

Reaction notes

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