NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

Participants

Reaction Conditions

Reaction Steps

An NADPH production system was prepared by mixing 42 mg of NADP, 5 ml of 100 mM glucose-6-phosphatase (G6P) and 5 ml of 100 mM MgCl2, and adding 57 μl of G6P dehydrogenase (about 1750 U/5 mg/ml) to the mixture. This was heated at 37° C. for 5 minutes and then ice-cooled until its use.

Provenance & Evidence