Sucrose → L-Lysine

Participants

Reaction Conditions

Reaction Steps

Strains of Brevibacterium lactofermentum AJ 12593 (FERM BP-3240) were inoculated in a culture medium comprising 80 g/L of waste molasses as a sucrose, 50 g/L of ammonium sulfate, 1 g/L of KH2PO4, 1 g/L of MgSO4.7H2O, 10 mg/L of soybean protein hydrolyzate (as nitrogen), 0.1 mg/L of thiamine hydrochloride, 50 g/L of calcium carbonate and 0.3 mg/L of biotin. The mixture was cultured while agitating at 31.5° C. for 72 hours to form a L-lysine fermentation broth. Then, the resultant fermentation broth was dried using a drum dryer to form 5 kg of a dried flaky product having the composition below.

Provenance & Evidence