Sucrose → L-Lysine

HRID 1584705Notes

Reaction equation

[CH2:1](O)[C@H:2]1[O:7][C@H:6]([O:8][C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H:5](O)[C@@H:4](O)[C@@H:3]1O.S([O-])([O-])(=O)=O.[NH4+:29].[NH4+:30].OP([O-])(O)=O.[K+].CC1[N+](CC2C=NC(C)=NC=2N)=CSC=1CCO.Cl.[Cl-].C(=O)([O-])[O-].[Ca+2].OC(CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=O>>[NH2:29][C@H:5]([C:6]([OH:8])=[O:7])[CH2:4][CH2:3][CH2:2][CH2:1][NH2:30]

Participants

Reactant 6

HCID 5988Sucrose

HCID 6202Thiamine Hydrochloride

HCID 10112Calcium Carbonate

HCID 171548Biotin

HCID 516951Monobasic potassium phosphate

HCID 6097028Ammonium Sulfate

Product 1

HCID 5962L-Lysine

Conditions

Temperature: 31.5 °C

Procedure

Strains of Brevibacterium lactofermentum AJ 12593 (FERM BP-3240) were inoculated in a culture medium comprising 80 g/L of waste molasses as a sucrose, 50 g/L of ammonium sulfate, 1 g/L of KH2PO4, 1 g/L of MgSO4.7H2O, 10 mg/L of soybean protein hydrolyzate (as nitrogen), 0.1 mg/L of thiamine hydrochloride, 50 g/L of calcium carbonate and 0.3 mg/L of biotin. The mixture was cultured while agitating at 31.5° C. for 72 hours to form a L-lysine fermentation broth. Then, the resultant fermentation broth was dried using a drum dryer to form 5 kg of a dried flaky product having the composition below.

Sources & evidence

ORD record: ord-7d3a380798b84fb294a1d263b84c4d01
Source dataset: uspto-grants-1999_08
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05935635

Reaction notes

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