5-Aminofluorescein → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

Beteiligte Stoffe

Reaktionsschritte

Alternatively, the product from step 1 can be reacted in the presence of carbodiimide with fluoresceinamine (FAM) (Reaction C) to provide 5-[2',3'-dideoxyinosin-5'-yl)-succinamido]-fluorescein (ddI-5'-S:FAM). An additional alternative is to react the product from step 2 with 5-[N'-(2-aminoethyl)-thioureido]fluorescein (fluoresceinthiocarbamylethylenediamine) (FTED) (Reaction D) to provide the tracer 5-[2-(2',3'-dideoxyinosin-5'-yl-succinamido)-ethylthiocarbamyl -fluorescein (ddI-5.-S:FTED).

Herkunft & Nachweise