反应详情

The hydrolysis of 1.60 g (5.12 mmol) of ethyl (RS)-α-[(tert-butylsulfonyl)methyl]hydrocinnamate was carried out in a manner analogous to Example 1 in the presence of 25 ml of aqueous 5 mM calcium chloride solution and 100 mg of Alcalase 2.0 T (NOVO, 2880 Bagsvaerd, Denmark). After a consumption of 2.47 ml of 1N sodium hydroxide solution (after 21.3 hours), the reaction mixture was worked-up analogously to Example 1, whereby there were obtained 700 mg (2.46 mmol, 48%, 96% of theory) of (S)-α-[(tert-butylsulfonyl)methyl]hydrocinnamic acid, which had not only an enantiomeric excess, but also a gas chromatographical (GC) purity of, in each case, >99%.

1. customAfter a consumption of 2.47 ml of 1N sodium hydroxide solution (after 21.3 hours)