Sulfuric acid magnesium salt (1:1) → L-Aspartic Acid

HRID 1589352Notes

Reaction equation

HRID 1589352 reaction equation
[C:1]([O-:8])(=[O:7])/[CH:2]=[CH:3]/[C:4]([O-:6])=[O:5].[NH4+:9].[NH4+].S([O-])([O-])(=O)=O.[Mg+2].SC(O)C>>[NH2:9][C@H:2]([C:1]([OH:8])=[O:7])[CH2:3][C:4]([OH:6])=[O:5]

Participants

4

Reactant 3

HCID 24083Sulfuric acid magnesium salt (1:1)HCID 4713212Mercapto-ethanolHCID 9793847Ammonium fumarate

Product 1

HCID 5960L-Aspartic Acid

Procedure

The stoichiometry of our immobilized aspartase, prepared as described in the previous examples, was determined using a 1 g column of immobilized aspartase, approximately 5,000 units per gram activity, operating at 40° C. The feedstock was 1.5 molar ammonium fumarate at pH 8.5 containing 10-3 molar each of magnesium sulfate and mercaptoethanol. The contents of the flask was circulated upflow through the jacketed column with the effluent being returned to the feed flask. The amount of fumaric acid consumed and aspartic acid formed was measured at various times using high-pressure liquid chromatography with results being summarized in Table 3.

Workup

  1. customThe stoichiometry of our immobilized aspartase, prepared
  2. customat 40° C
  3. customThe amount of fumaric acid consumed

Sources & evidence

ORD record
ord-ba450b71edda4cbe94064041f3c06541
Source dataset
uspto-grants-1986_02
Patent number
US04569911

Reaction notes

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