Sulfuric acid magnesium salt (1:1) → L-Aspartic Acid

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Reaction Steps

The stoichiometry of our immobilized aspartase, prepared as described in the previous examples, was determined using a 1 g column of immobilized aspartase, approximately 5,000 units per gram activity, operating at 40° C. The feedstock was 1.5 molar ammonium fumarate at pH 8.5 containing 10-3 molar each of magnesium sulfate and mercaptoethanol. The contents of the flask was circulated upflow through the jacketed column with the effluent being returned to the feed flask. The amount of fumaric acid consumed and aspartic acid formed was measured at various times using high-pressure liquid chromatography with results being summarized in Table 3.

Provenance & Evidence