反应详情

Equimolar amounts of chromium chloride hexahydrate and ATP (10 mM each) were mixed in 50 ml of water at a pH of around 3. The mixture was then incubated at room temperature for 1-2 hours to form the chromium-ATP complex. This complex was purified by loading 50 ml of the 10 mM chromium-ATP mixture onto a SE53 ion-exchange column having a bed volume of approximately 30 ml. The loaded column was washed with 100 ml of water in order to wash off unbound materials. The bound chromium complex was then eluted with 0.25 M ammonium hydroxide. The eluted fractions (4 ml/fraction) were monitored with a UV spectrophotometer for absorption at wavelengths A260 and A280. The peak fraction (the one with the highest absorbance) was collected and lyophilized.

1. customto form the chromium-ATP complex
2. customThis complex was purified
3. washThe loaded column was washed with 100 ml of water in order
4. washto wash off unbound materials
5. washThe bound chromium complex was then eluted with 0.25 M ammonium hydroxide
6. customThe eluted fractions (4 ml/fraction) were monitored with a UV spectrophotometer for absorption at wavelengths
7. customThe peak fraction (the one with the highest absorbance) was collected