Water → HCID 178437364, compound

HRID 1597070Notes

Reaction equation

[CH2:1](O)[C:2](N)(CO)CO.Cl.[C@@H:10]1([N:19]2[C:28]3[N:27]=[CH:26][N:25]=[C:23](N)[C:22]=3[N:21]=[CH:20]2)[O:18][C@H:15]([CH2:16][OH:17])[C@@H:13]([OH:14])[C@H:11]1O.[OH2:29]>>[C:1]([C@:15]1([CH2:16][OH:17])[O:18][C@@H:10]([N:19]2[C:28]3[N:27]=[CH:26][N:25]=[C:23]([OH:29])[C:22]=3[N:21]=[CH:20]2)[CH2:11][C@@H:13]1[OH:14])#[CH:2]

Participants

Reactant 3

HCID 962Water

HCID 60961Adenosine

HCID 93573Tromethamine hydrochloride

Product 1

HCID 178437364HCID 178437364, compound

Conditions

Temperature: 40 °C

Procedure

To a Tris-HCl buffer solution (6 ml, pH 7.5) of Compound 23 (0.022 g, 0.08 mmol), adenosine deaminase (0.044 ml, 20 unit) was added, and the mixture was stirred for 2.5 hours at 40° C., followed by cooling to room temperature. The reaction mixture was applied to a reverse-phase ODS silica gel column (50 g), desalted by water (500 ml) flow, and through use of a 2.5% aqueous ethanol, Compound 29 was eluted. Subsequently, the Compound 29 was pulverized with isopropanol, to thereby yield 0.016 g of Compound 29 (72%).

Workup

temperatureby cooling to room temperature
washthrough use of a 2.5% aqueous ethanol, Compound 29 was eluted

Sources & evidence

ORD record: ord-421d2be30aac4743b967cbf7a33c2f0c
Source dataset: uspto-grants-2001_09
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US06291670B1

Reaction notes

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