反应详情

(E)-(6R,7R)-7-(2-bromo-acetylamino)-3-(1-cyclopropyl-2-oxo-pyrrolidin-3-ylidenemethyl)-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid sodium salt (1:1) (see ex. C4a) (200 mg, 0.42 mmol) was dissolved in 4 ml N,N-dimethylformamide and 2-mercaptobenzimidazole sodium salt (79.2 mg, 0.46 mmol) was added in a single portion. After completion of the reaction, the solvent was stripped off at a rotary evaporator. The residue was dissolved in water and chromatographed on MCI gel (75-150μ, Mitsubishi Kasei Corporation) with a gradient of water:acetonitril (10:1, 9:1). The organic solvent was evaporated and the aqueous phase was freeze-dried.

1. customAfter completion of the reaction
2. customthe solvent was stripped off at a rotary evaporator
3. dissolutionThe residue was dissolved in water
4. customchromatographed on MCI gel (75-150μ, Mitsubishi Kasei Corporation) with a gradient of water:acetonitril (10:1, 9:1)
5. customThe organic solvent was evaporated
6. customthe aqueous phase was freeze-dried