Isopropanol → HCID 131710129, compound

HRID 1604925Notes

Reaction equation

HRID 1604925 reaction equation
Cl[CH:2](Cl)[C:3]([O:5][CH2:6][C:7]1[CH2:8][S:9][C@@H:10]2[C@H:17]([NH:18][C:19](=[O:26])[CH2:20][C:21]3[S:22][CH:23]=[CH:24][CH:25]=3)[C:16](=[O:27])[N:11]2[C:12]=1[C:13]([OH:15])=[O:14])=O.[CH:29](O)(C)C>>[CH:3]([O:5][CH2:6][C:7]1[CH2:8][S:9][C@@H:10]2[C@H:17]([NH:18][C:19](=[O:26])[CH2:20][C:21]3[S:22][CH:23]=[CH:24][CH:25]=3)[C:16](=[O:27])[N:11]2[C:12]=1[C:13]([OH:15])=[O:14])([CH3:29])[CH3:2]

Participants

3

Reactant 2

HCID 3776IsopropanolHCID 131710126HCID 131710126, compound

Product 1

HCID 131710129HCID 131710129, compound

Procedure

3-Dichloroacetoxymethyl-7β-(2'-thienylacetamido)-ceph-3-em-4-carboxylic acid (12.0 g., 25.8 m.mole.) was refluxed in isopropanol (100 ml.) for 45 minutes. After filtration through a pad of Kieselguhr, the isopropanol solution was poured into water (1000 ml.) and the pH adjusted to 8.5. The solution was extracted with ethyl acetate (2 × 100 ml.) and the extracts discarded. The solution was then acidified to pH 1.5 with 2N-hydrochloric acid and extracted with ethyl acetate (3 × 100 ml.). The ethyl acetate extracts were dried over magnesium sulphate and evaporated. The residue (8.5 g.) was dissolved in a mimimum of hot ethanol, from which the title compound separated on cooling (1.5 g., 15%). This material recrystallised from ethanol as colourless prisms, m.p. 169°-171° (d), [α]D28 + 87° (c 1.0, tetrahydrofuran), λλmax (ethanol) 237 nm (ε 14,500), 260 nm (ε 7,900), νmax (Nujol) 1775 (β-lactam), 1728 (COOH), 1668 and 1535 cm.-1 (CONH), N.M.R. (D2O, with sodium bicarbonate) 5.64 and 5.95 (quartet: J = 16 Hz, -CH2OCH(CH3)2), 6.31, 8.87τ )-CH2 -OCH (CH3)2 respectively). (Found: C, 51.6; H, 5.1; N, 6.7; S, 16.3. C17H20N2O5S2 requires C, 51.5; H, 5.1; N, 6.1; S, 16.2%). Rf 0.32 (system B), 0.65 (system C).

Workup

  1. filtrationAfter filtration through a pad of Kieselguhr
  2. additionthe isopropanol solution was poured into water (1000 ml.)
  3. extractionThe solution was extracted with ethyl acetate (2 × 100 ml.)
  4. extractionextracted with ethyl acetate (3 × 100 ml.)
  5. dry with materialThe ethyl acetate extracts were dried over magnesium sulphate
  6. customevaporated
  7. dissolutionThe residue (8.5 g.) was dissolved in a mimimum of hot ethanol, from which the title compound
  8. customseparated
  9. temperatureon cooling (1.5 g., 15%)
  10. customThis material recrystallised from ethanol as colourless prisms, m.p. 169°-171° (d), [α]D28 + 87° (c 1.0, tetrahydrofuran), λλmax (ethanol) 237 nm (ε 14,500), 260 nm (ε 7,900), νmax (Nujol) 1775 (β-lactam), 1728 (COOH), 1668 and 1535 cm

Sources & evidence

ORD record
ord-355060559a0641c68d3e858e64182eeb
Source dataset
uspto-grants-1976_04
Patent number
US03948906

Reaction notes

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