Acetate → HCID 178527090, compound

Participants

Reaction Steps

Cells of Escherichia coli JM109 introduced with each of the aforementioned mutant EB-AP genes were added at a density of 100 mg/dl in terms of dry cell weight to a solution in an acetate buffer (pH 4.0) containing 12 g/dl of pyrophosphoric acid and 6 g/dl of inosine, and reaction was performed at 30° C. for 24 hours while pH was maintained at 4.0. The results of measurement of the produced amount of 5′-inosinic acid are shown in Table 4. The produced inosinic acid consisted only of 5′-inosinic acid, and 2′-inosinic acid and 3′-inosinic acid byproducts were not observed at all.

Provenance & Evidence