Bullatacin → HCID 131725266, compound

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Reaction Steps

Insect antifeedant bioassay of squamocin-G and isosquamocin were found to be toxic to the first and third instar larvae of origental armyworm (Mythimna separata WLK) at a concentration of 10.05% (MeOH) at 27° causing 70% mortality compared to 7% mortality of untreated control. The sample solutions (10 μl of a 0.05% solution) were applied evenly to the lower surface of leaf discs (5 cm2) cut from sorghum hybrid variety CSH5 and the discs were allowed to dry on filter papers. After drying the leaf, discs were offered to 10 first instar M. separata larvae in plastic cups or to one third instar larva in 9-cm (diameter) plastic petric dishes. All larvae were starved for 2 h before the test and the leaf and the leaf discs were kept on moisture filter papers to keep them turgid. The larvae were confined to leaf discs for 24 h and during the period the larvae scratched or consumed >80% of the leaf disc area in the untreated controls. The leaf discs were rated visually for the extent of feeding on a scale upto 1 to 9 (1=<10%, 2=11 to 20%, 3=21 to 30%, 4=31 to 40%, 5=41 to 7=61 to 70%, 8=71 to 80%, 9=>80% leaf area scratched consumed). The larvae were then stirred for 12 h and weighed. Isosquamocin was evaluated by this procedure and the results have shown that it drastically. The feeding of the leaves of sorghum hybrid variety CSH 5 at a concentration of 0.05% in methanol) in comparison with control (methanol) against the first instar and third instar larvae of oriental armyworm (Mythimna separata WLK). It is very toxic to first instar larvae causing high larval mortality and the results are presented below:

Provenance & Evidence