HCID 54479933, compound → HCID 453628, compound

HRID 1613145Notes

Reaction equation

HRID 1613145 reaction equation
P([O:5][CH2:6][C@@:7]1([CH2:22][N:23]=[N+:24]=[N-:25])[O:11][C@@H:10]([N:12]2[CH:20]=[C:18]([CH3:19])[C:16](=[O:17])[NH:15][C:13]2=[O:14])[CH2:9][C@@H:8]1[OH:21])(O)(O)=O>O>[N:23]([CH2:22][C@:7]1([CH2:6][OH:5])[O:11][C@@H:10]([N:12]2[CH:20]=[C:18]([CH3:19])[C:16](=[O:17])[NH:15][C:13]2=[O:14])[CH2:9][C@@H:8]1[OH:21])=[N+:24]=[N-:25]

Participants

3

Reactant 1

HCID 54479933HCID 54479933, compound2×

Product 1

HCID 453628HCID 453628, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Sufficient water is added to 100 g of egg-yolk phospholipids to bring the total volume to 1 liter. The mixture is stirred with a homomixer. Then, the mixture is homogenized with an emulsifier under a pressure of 300 kg/cm2 for 30 minutes, whereby an aqueous phospholipid dispersion is obtained 4'-Azidomethylthymidine 5'-monophosphate (20 g) and sodium chloride (18 g) are dissolved in enough water to bring the total volume to 1 liter. The aqueous phospholipid dispersion (850 mL) and the 4'-azidomethylthymidine 5'-monophosphate solution (850 mL) are mixed. The aqueous dispersion thus obtained is filtered through a membrane filter (pore size 0.45 m in diameter). The filtrate is sterilized at 120° C. for 20 minutes and then allowed to stand at -20° C. for 20 hours in a freezer. The frozen dispersion thus obtained is thawed by allowing it to stand at room temperature. An aqueous suspension of 4'-azidomethylthymidine 5'-monophosphate entrapped in phospholipid spherules is thereby obtained.

Workup

  1. customThe aqueous dispersion thus obtained
  2. filtrationis filtered through a membrane
  3. filtrationfilter (pore size 0.45 m in diameter)
  4. waitto stand at -20° C. for 20 hours in a freezer
  5. customThe frozen dispersion thus obtained
  6. customto stand at room temperature
  7. customis thereby obtained

Sources & evidence

ORD record
ord-5012331bb5db402f8b417066bffcb3f1
Source dataset
uspto-grants-1993_03
Patent number
US05192749

Reaction notes

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