HCID 54479933, compound → HCID 453628, compound

Participants

Reaction Steps

Sufficient water is added to 100 g of egg-yolk phospholipids to bring the total volume to 1 liter. The mixture is stirred with a homomixer. Then, the mixture is homogenized with an emulsifier under a pressure of 300 kg/cm2 for 30 minutes, whereby an aqueous phospholipid dispersion is obtained 4'-Azidomethylthymidine 5'-monophosphate (20 g) and sodium chloride (18 g) are dissolved in enough water to bring the total volume to 1 liter. The aqueous phospholipid dispersion (850 mL) and the 4'-azidomethylthymidine 5'-monophosphate solution (850 mL) are mixed. The aqueous dispersion thus obtained is filtered through a membrane filter (pore size 0.45 m in diameter). The filtrate is sterilized at 120° C. for 20 minutes and then allowed to stand at -20° C. for 20 hours in a freezer. The frozen dispersion thus obtained is thawed by allowing it to stand at room temperature. An aqueous suspension of 4'-azidomethylthymidine 5'-monophosphate entrapped in phospholipid spherules is thereby obtained.

Provenance & Evidence