Trifluoroacetic acid → HCID 178474246, compound

HRID 1621656Notes

Reaction equation

[Cl:1][C:2]1[CH:7]=[C:6]([NH:8][C:9]2[C:14]([C:15]3[N:23]=[C:22]([CH3:24])[N:21]=[C:20]4[C:16]=3[N:17]=[CH:18][N:19]4C3CCCCO3)=[CH:13][CH:12]=[CH:11][N:10]=2)[CH:5]=[CH:4][C:3]=1[NH:31][C:32](=[O:34])[CH3:33].FC(F)(F)C(O)=O>C(Cl)Cl>[Cl:1][C:2]1[CH:7]=[C:6]([NH:8][C:9]2[C:14]([C:15]3[N:23]=[C:22]([CH3:24])[N:21]=[C:20]4[C:16]=3[N:17]=[CH:18][NH:19]4)=[CH:13][CH:12]=[CH:11][N:10]=2)[CH:5]=[CH:4][C:3]=1[NH:31][C:32](=[O:34])[CH3:33]

Participants

Conditions

Temperature: 0 °C

Procedure

A mixture of N-(2-chloro-4-(3-(2-methyl-9-(tetrahydro-2H-pyran-2-yl)-9H-purin-6-yl)pyridin-2-ylamino)phenyl)acetamide (100 mg, 0.209 mmol) in DCM (3 mL) was cooled to 0° C., treated with trifluoroacetic acid (3 mL, 40.4 mmol), and the yellow solution was stirred at 0° C. for 1 h. The mixture was concentrated in vacuo, the residue was treated with 2 M NH3 in MeOH and concentrated in vacuo. The crude product was purified by chromatography through a Redi-Sep pre-packed silica gel column (40 g), eluting with a gradient of 2% to 10% 2M NH3-MeOH in DCM, to give N-(2-chloro-4-(3-(2-methyl-9H-purin-6-yl)pyridin-2-ylamino)phenyl)acetamide (70 mg, 0.178 mmol, 58.3% yield) as a yellow solid. 1H NMR (400 MHz, d6-DMSO) δ 13.62 (s, 1H); 13.01 (s, 1H); 9.80 (s, 1H); 9.47 (s, 1H); 8.38-8.43 (m, 1H); 8.31 (d, J=1.57 Hz, 1H); 7.54-7.60 (m, 1H); 7.47-7.52 (m, 1H); 7.07 (dd, J=7.82, 4.69 Hz, 1H); 2.88 (s, 3H); 2.07 (s, 3H). m/z (ESI, +ve ion) 394 (M+H)+.

Workup

concentrationThe mixture was concentrated in vacuo
additionthe residue was treated with 2 M NH3 in MeOH
concentrationconcentrated in vacuo
customThe crude product was purified by chromatography through a Redi-Sep pre-packed silica gel column (40 g)
washeluting with a gradient of 2% to 10% 2M NH3-MeOH in DCM

Sources & evidence

ORD record: ord-a1e17cfea18441258c9f310e5e971e8a
Source dataset: uspto-grants-2014_07
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US08772480B2

Reaction notes

Loading…