反应详情

150 mg (0.39 mmol) of 2,6-dimethyl-8-[4,4,4-trifluoro-3-(trifluoromethyl)butoxy]imidazo[1,2-a]-pyridine-3-carboxylic acid Example 91A and 163 mg (0.43 mmol) of HATU were initially charged in 2.5 ml of DMF. 0.2 ml (1.17 mmol) of N,N-diisopropylethylamine and 50.3 mg (0.43 mmol) of (R)-(−)-2-amino-1-hexanol were then added, and the mixture was stirred at RT overnight. 10 ml of water were added, the reaction solution was extracted twice with 20 ml of ethyl acetate and the combined organic phases were washed with 20 ml of water and with 20 ml of saturated aqueous sodium chloride solution, dried over magnesium sulphate, filtered and concentrated. The residue was purified by preparative RP-HPLC (acetonitrile-water+0.1% formic acid), the product fractions were combined and concentrated to dryness and the residue was dissolved in a mixture of 1.5 ml of tert-butanol and 2 ml of water and lyophilized overnight. This gave 42 mg of the target compound (95% pure, 21% of theory).

1. extractionthe reaction solution was extracted twice with 20 ml of ethyl acetate
2. washthe combined organic phases were washed with 20 ml of water and with 20 ml of saturated aqueous sodium chloride solution
3. dry with materialdried over magnesium sulphate
4. filtrationfiltered
5. concentrationconcentrated
6. customThe residue was purified by preparative RP-HPLC (acetonitrile-water+0.1% formic acid)
7. concentrationconcentrated to dryness
8. dissolutionthe residue was dissolved in a mixture of 1.5 ml of tert-butanol and 2 ml of water
9. waitlyophilized overnight