Glycine → Ethylenediaminetetraacetic Acid

Participants

Role	Name	HCID	Charge
Reactant	Glycine	HCID 750	—
Reactant	EDTA disodium salt	HCID 8759	—
Reactant	(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal	HCID 107526	—
Solvent	Water	HCID 962	—
Product	Glycine	HCID 750	—
Product	Ethylenediaminetetraacetic Acid	HCID 6049	1%
Product	(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal	HCID 107526	—

Reaction Steps

A 10% (volume/volume) Tween 80 solution was prepared by adding 10 ml Tween 80 to 90 ml H2O. A 1% (weight/volume) EDTA solution was prepared by adding 1 g Na2EDTA to 100 ml H2O. A 0.1 M glycine solution was prepared by adding 1.5 g glycine (M. W. 75) to 200 ml H2O. A 0.5% w/v) hydroxypropyl-methyl-cellulose (HPMC, 100 centipoise) solution was prepare by adding 1 g EPMC to 200 ml H2O and gently mixing until fully dissolved. A 250 unit/ml GOX (Type VII from Aspergillus niger, Sigma Chemicals) was prepared in H2O. A 0.1 M glucose solution was prepared by adding 1.8 g D-glucose (M. W. 180) to 100 ml H2O.

Provenance & Evidence

DOI	10.6084/m9.figshare.5104873.v1
Patent number	US06503507B1
Source dataset	uspto-grants-2003_01
ORD ID	ord-595c58429d50407b80ea91d3324d9920
Source note	uspto-grants-2003_01