反应详情

A 10% (volume/volume) Tween 80 solution was prepared by adding 10 ml Tween 80 to 90 ml H2O. A 1% (weight/volume) EDTA solution was prepared by adding 1 g Na2EDTA to 100 ml H2O. A 0.1 M glycine solution was prepared by adding 1.5 g glycine (M. W. 75) to 200 ml H2O. A 0.5% w/v) hydroxypropyl-methyl-cellulose (HPMC, 100 centipoise) solution was prepare by adding 1 g EPMC to 200 ml H2O and gently mixing until fully dissolved. A 250 unit/ml GOX (Type VII from Aspergillus niger, Sigma Chemicals) was prepared in H2O. A 0.1 M glucose solution was prepared by adding 1.8 g D-glucose (M. W. 180) to 100 ml H2O.

1. customwas prepare
2. additiongently mixing
3. dissolutionuntil fully dissolved