Diisopropylethylamine → 7-(2-Amino-4-methyl-1H-benzo[d]imidazol-6-yl)-1-((tetrahydro-2H-pyran-4-yl)Methyl)-3,4-dihydropyrazino[2,3-b]pyrazin-2(1H)-one

HRID 1640299Notes

Reaction equation

HRID 1640299 reaction equation
Br[C:2]1[N:3]=[C:4]([NH:15][CH2:16][CH:17]2[CH2:22][CH2:21][O:20][CH2:19][CH2:18]2)[C:5]([NH:8][CH2:9][C:10]([O:12]CC)=O)=[N:6][CH:7]=1.BrC1[C:25]([NH:31]CC(OCC)=O)=[N:26][CH:27]=[C:28](Br)N=1.O1[CH2:43][CH2:42][CH:41]([CH2:44][NH2:45])[CH2:40]C1.C(N(CC)C(C)C)(C)C>CO.CS(C)=O>[NH2:31][C:25]1[NH:26][C:27]2[CH:28]=[C:43]([C:2]3[N:3]=[C:4]4[N:15]([CH2:16][CH:17]5[CH2:18][CH2:19][O:20][CH2:21][CH2:22]5)[C:10](=[O:12])[CH2:9][NH:8][C:5]4=[N:6][CH:7]=3)[CH:42]=[C:41]([CH3:40])[C:44]=2[N:45]=1

Participants

7

Reactant 4

HCID 81531DiisopropylethylamineHCID 27732104-AminomethyltetrahydropyranHCID 57550751Ethyl 2-(3,5-dibromopyrazin-2-ylamino)acetateHCID 86644641Ethyl 2-(5-bromo-3-((tetrahydro-2H-pyran-4-yl)methylamino)pyrazin-2-ylamino)acetate

Product 1

HCID 582983117-(2-Amino-4-methyl-1H-benzo[d]imidazol-6-yl)-1-((tetrahydro-2H-pyran-4-yl)Methyl)-3,4-dihydropyrazino[2,3-b]pyrazin-2(1H)-one

Reagents, catalysts & solvents 2

HCID 679Dimethyl SulfoxideSolventHCID 887MethanolSolvent

Conditions

Temperature
150 °C

Procedure

Ethyl 2-(5-bromo-3-((tetrahydro-2H-pyran-4-yl)methylamino)pyrazin-2-ylamino)acetate. Ethyl 2-(3,5-dibromopyrazin-2-ylamino)acetate (2.00 g, 5.90 mmol), (tetrahydro-2H-pyran-4-yl)methanamine (0.713 g, 6.19 mmol), N,N-diisopropylethylamine (3.08 mL, 17.70 mmol) and dimethylsulfoxide (4 mL) were combined in a microwave vial with a stirbar and heated in a Biotage Emrys Optimizer microwave reactor at 150° C. for 1 h. The resulting mixture was transferred to a round bottom flask with methanol. The methanol and N,N-diisopropylethylamine were removed under reduced pressure and the residue purified using Biotage flash chromatography (5-100% ethyl acetate in hexane). Fractions containing the desired product were combined in a separatory funnel and washed twice with water and once with brine. The organics were dried over magnesium sulfate, filtered and concentrated under reduced pressure. The residue was dried under high vacuum at 50° C. to give impure desired product (1.578 g) as an amber waxy solid which was taken on to the next step without further purification. MS (ESI) m/z 373.4 [M]+, 375.4 [M+2]+.

Workup

  1. customThe methanol and N,N-diisopropylethylamine were removed under reduced pressure
  2. customthe residue purified
  3. additionFractions containing the desired product
  4. customwere combined in a separatory funnel
  5. washwashed twice with water and once with brine
  6. dry with materialThe organics were dried over magnesium sulfate
  7. filtrationfiltered
  8. concentrationconcentrated under reduced pressure
  9. customThe residue was dried under high vacuum at 50° C.

Sources & evidence

ORD record
ord-1df4392fe91f43b59882fba4de7ca668
Source dataset
uspto-grants-2012_02
Patent number
US08110578B2

Reaction notes

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