(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → D-(-)-Fructose

Participants

Reaction Steps

A 50% solution of cornstarch hydrolyzate containing dextrose on a dry solids basis, 5 mM MgSo4 and 5 mM NaHSO3 was adjusted in pH to 7.8, and started downflow through the bed of immobilized isomerase at 61° C. Initial flow through the column was calculated from equation 1 and was set to provide a fructose conversion of about 44%. This flow of about 17 ml/hour was held constant except during assay of immobilized activity. Fructose content of the effluent was measured essentially by the method as for determining degree of isomerization.

Provenance & Evidence