(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → D-(-)-Fructose

HRID 1645769Notes

Reaction equation

[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].OS([O-])=O.[Na+]>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

Reactant 2

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HCID 23665763Sodium Bisulfite

Product 1

HCID 5984D-(-)-Fructose

Procedure

A 50% solution of cornstarch hydrolyzate containing dextrose on a dry solids basis, 5 mM MgSo4 and 5 mM NaHSO3 was adjusted in pH to 7.8, and started downflow through the bed of immobilized isomerase at 61° C. Initial flow through the column was calculated from equation 1 and was set to provide a fructose conversion of about 44%. This flow of about 17 ml/hour was held constant except during assay of immobilized activity. Fructose content of the effluent was measured essentially by the method as for determining degree of isomerization.

Workup

customstarted downflow through the bed of immobilized isomerase at 61° C

Sources & evidence

ORD record: ord-dc76d4bc70e449d39f47453f8bf3ea25
Source dataset: uspto-grants-1993_01
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05177005

Reaction notes

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