Trifluoroacetate → L-Glutamic Acid

HRID 1647905Notes

Reaction equation

HRID 1647905 reaction equation
C[C@H]([NH:36][C:37]([CH2:39][CH2:40][C:41]([OH:43])=[O:42])=O)C(N[C@H](C(N1[C@H](C(N[C@H](C(SCC2C=CC=CC=2)=O)CC2C=CC=CC=2)=O)CCC1)=O)C)=O.FC(F)(F)[C:46]([O-:48])=[O:47]>>[NH2:36][C@H:37]([C:46]([OH:48])=[O:47])[CH2:39][CH2:40][C:41](=[O:42])[OH:43]

Participants

3

Reactant 2

HCID 84468TrifluoroacetateHCID 90477353HCID 90477353, compound

Product 1

HCID 33032L-Glutamic Acid

Procedure

First, 10 g of carboxypeptidase B (Sigma) was added to 100 pl of a solution of the (Glu-OC)-Lys monomers obtained in the above step (6), and the mixture was left for 2 hr at 37° C. Then the reaction was stopped by the addition of 50.1 of 0.1% trifluoroacetate solution. The mixture was separated by high-pressure liquid chromatography with use of a C18 reversed-phase column, and Glu-OC was obtained quantitatively.

Workup

  1. customobtained in the above step (6)
  2. customThe mixture was separated by high-pressure liquid chromatography with use of a C18 reversed-phase column

Sources & evidence

ORD record
ord-959c905d4762469683eb1e16a659a834
Source dataset
uspto-grants-1995_07
Patent number
US05434245

Reaction notes

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