Trifluoroacetate → L-Glutamic Acid

Participants

Reaction Steps

First, 10 g of carboxypeptidase B (Sigma) was added to 100 pl of a solution of the (Glu-OC)-Lys monomers obtained in the above step (6), and the mixture was left for 2 hr at 37° C. Then the reaction was stopped by the addition of 50.1 of 0.1% trifluoroacetate solution. The mixture was separated by high-pressure liquid chromatography with use of a C18 reversed-phase column, and Glu-OC was obtained quantitatively.

Provenance & Evidence