Hydrochloric Acid → HCID 18649444, compound

A methanol solution (75 ml) containing (2R,3S)-2-(2,4-difluorophenyl)-2-(1-imidazolyl)methyl-3-methyloxirane (2.5 g), methyl 3-mercaptopropionate (5.5 ml) and 28% sodium methoxide-methanol (8.1 ml) was refluxed for 1.5 hours. Then, methyl 3-mercaptopropionate (5.5 ml) and 28% sodium methoxide-methanol (8.1 ml) were added and the mixture was further refluxed for 2 hours. The reaction mixture was cooled with ice, neutralized with 5N hydrochloric acid (16 ml), diluted with saturated aqueous sodium chloride solution (100 ml) and extracted with ethyl acetate (200 ml×3). The extract was dried over anhydrous magnesium sulfate and the solvent was distilled off under reduced pressure. The residue was dissolved in methylene chloride (40 ml), followed by addition of 1N aqueous sodium hydroxide solution (8 ml) and water (100 ml). The aqueous layer was further extracted with methylene chloride (40 ml×3). The methylene chloride layers were combined and extracted 5 times with water (30 ml) containing 1N hydrochloric acid (8 ml). The aqueous layers were combined, neutralized with sodium hydroxide and extracted with methylene chloride (40 ml×4). The extract was dried over anhydrous magnesium sulfate and the solvent was distilled off under reduced pressure. The residue was subjected to silica gel chromatography (4 cm×15 cm) using methanol-methylene chloride (5:95) as the eluent. The desired fraction was concentrated and diethyl ether was added to the residue to yield (2R,3R)-2-(2,4-difluorophenyl)-1-(1-imidazolyl)-3-mercapto-2-butanol (0.84 g) as colorless prisms.

1. temperaturewas refluxed for 1.5 hours
2. temperaturethe mixture was further refluxed for 2 hours
3. temperatureThe reaction mixture was cooled with ice
4. extractionextracted with ethyl acetate (200 ml×3)
5. dry with materialThe extract was dried over anhydrous magnesium sulfate
6. distillationthe solvent was distilled off under reduced pressure
7. dissolutionThe residue was dissolved in methylene chloride (40 ml)
8. additionfollowed by addition of 1N aqueous sodium hydroxide solution (8 ml) and water (100 ml)
9. extractionThe aqueous layer was further extracted with methylene chloride (40 ml×3)
10. extractionextracted 5 times with water (30 ml)
11. additioncontaining 1N hydrochloric acid (8 ml)
12. extractionextracted with methylene chloride (40 ml×4)
13. dry with materialThe extract was dried over anhydrous magnesium sulfate
14. distillationthe solvent was distilled off under reduced pressure
15. concentrationThe desired fraction was concentrated
16. additiondiethyl ether was added to the residue

ORD record

ord-ee9116f034d44f03a04b370e681e4351

Source dataset

uspto-grants-1995_04

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05405861