Nadide (JAN/USAN/INN) → NADPH

Participants

Reaction Conditions

Reaction Steps

To 0.9 ml of each of the substrate solutions containing NADH or NAD+, respectively, shown in Table 1 was added 0.1 ml of 23 U/ml of the present enzyme, and the reaction was carried out at 30° C. for 20 minutes. Then, the reaction was terminated by heat treatment at 100° C. for 2 minutes, and the denatured protein was removed, after which the amount of NADPH or NADP+ produced by the reaction of the corresponding substrate was measured as follows. As a control solution, there was used a mixture of each substrate solution and the present enzyme in which the reaction had been terminated immediately after mixing them.

Provenance & Evidence