Nadide (JAN/USAN/INN) → NADPH

HRID 1655158Notes

Reaction equation

[CH:1]1[N:2]=[C:3]([NH2:44])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][CH2:25][C@H:26]4[O:30][C@@H:29]([N:31]5[CH:36]=[C:35]([C:37]([NH2:39])=[O:38])[CH2:34][CH:33]=[CH:32]5)[C@H:28]([OH:40])[C@@H:27]4[OH:41])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:42])[C@H:11]3[OH:43])[C:5]=2[N:6]=1.[CH:45]1[CH:50]=[N+:49]([C@@H:51]2[O:55][C@H:54]([CH2:56][O:57][P:58]([O:61][P:62]([O:65][CH2:66][C@H:67]3[O:71][C@@H:70]([N:72]4[C:76]5[N:77]=[CH:78][N:79]=[C:80]([NH2:81])[C:75]=5[N:74]=[CH:73]4)[C@H:69]([OH:82])[C@@H:68]3[OH:83])([OH:64])=[O:63])([OH:60])=[O:59])[C@@H:53]([OH:84])[C@H:52]2[OH:85])[CH:48]=[C:47]([C:86]([NH2:88])=[O:87])[CH:46]=1>>[CH:1]1[N:2]=[C:3]([NH2:44])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][CH2:25][C@H:26]4[O:30][C@@H:29]([N:31]5[CH:36]=[C:35]([C:37]([NH2:39])=[O:38])[CH2:34][CH:33]=[CH:32]5)[C@H:28]([OH:40])[C@@H:27]4[OH:41])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:42])[C@H:11]3[O:43][P:58]([OH:60])([OH:59])=[O:57])[C:5]=2[N:6]=1.[CH:45]1[CH:50]=[N+:49]([C@@H:51]2[O:55][C@H:54]([CH2:56][O:57][P:58]([O:61][P:62]([O:65][CH2:66][C@H:67]3[O:71][C@@H:70]([N:72]4[C:76]5[N:77]=[CH:78][N:79]=[C:80]([NH2:81])[C:75]=5[N:74]=[CH:73]4)[C@H:69]([O:82][P:17]([OH:19])([OH:18])=[O:16])[C@@H:68]3[OH:83])([OH:64])=[O:63])([OH:60])=[O:59])[C@@H:53]([OH:84])[C@H:52]2[OH:85])[CH:48]=[C:47]([C:86]([NH2:88])=[O:87])[CH:46]=1

Participants

Reactant 2

HCID 5893Nadide (JAN/USAN/INN)

HCID 439153Nadh

Product 2

HCID 5884NADPH

HCID 5886NADP nicotinamide-adenine-dinucleotide phosphate

Conditions

Temperature: 100 °C

Procedure

To 0.9 ml of each of the substrate solutions containing NADH or NAD+, respectively, shown in Table 1 was added 0.1 ml of 23 U/ml of the present enzyme, and the reaction was carried out at 30° C. for 20 minutes. Then, the reaction was terminated by heat treatment at 100° C. for 2 minutes, and the denatured protein was removed, after which the amount of NADPH or NADP+ produced by the reaction of the corresponding substrate was measured as follows. As a control solution, there was used a mixture of each substrate solution and the present enzyme in which the reaction had been terminated immediately after mixing them.

Workup

customThen, the reaction was terminated
customthe denatured protein was removed

Sources & evidence

ORD record: ord-b99030369a6947cc9af0d983a6926111
Source dataset: uspto-grants-1993_10
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05250416

Reaction notes

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