Phosphate → Indole

HRID 1656886Notes

Reaction equation

HRID 1656886 reaction equation
P([O-])([O-])([O-])=O.N[C@H](C(O)=O)C[C:9]1[C:17]2[C:12](=[CH:13][CH:14]=[CH:15][CH:16]=2)[NH:11][CH:10]=1>>[NH:11]1[C:12]2[C:17](=[CH:16][CH:15]=[CH:14][CH:13]=2)[CH:9]=[CH:10]1

Participants

3

Reactant 2

HCID 1061PhosphateHCID 6305L-Tryptophan

Product 1

HCID 798Indole

Procedure

0.1 ml of the aforesaid treated cell suspension was added to 25 ml of a reaction solution which contained per ml thereof, 100μmole of phosphate buffer (pH 8.0), 5 μmole of L-tryptophan and 0.04μmole of pyridoxalphosphoric acid, and reacted at 37° C. for 15 minutes. The amount of indole formed was determined by a conventional method [O. H. Smith and C. Janofsky: "Methods in Enzymology", Academic, New York, (1962, vol. 5, pages 794-806)].

Workup

  1. addition0.1 ml of the aforesaid treated cell suspension

Sources & evidence

ORD record
ord-000645fb627047f7b8b2e401957abe9f
Source dataset
uspto-grants-1993_10
Patent number
US05256551

Reaction notes

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