反应详情

温度

60 °C

In water (30 ml) were dissolved 7β-amino-3-(3-oxobutyryloxy)methyl-3-cephem-4-carboxylic acid (3.14 g), sodium hydrogen carbonate (1.84 g) and 5-mercapto-1-methyl-1H-tetrazole (1.4 g) and the solution was adjusted its pH to 5.5, followed by stirring and heating at 60° C. for one hour. After cooling, the reaction solution was washed with dichloromethane (20 ml) and the aqueous layer was adjusted to pH 3.3, followed by stirring for one hour under ice-cooling. The resultant precipitates were collected by filtration and washed with water, methanol and acetone in this order, followed by drying to give 7β-amino-3-(1-methyl-1H-tetrazol-5-yl)thiomethyl-3-cephem-4-carboxylic acid (2.7 g).

1. temperatureAfter cooling
2. washthe reaction solution was washed with dichloromethane (20 ml)
3. stirringby stirring for one hour under ice-
4. temperaturecooling
5. customThe resultant precipitates
6. filtrationwere collected by filtration
7. washwashed with water, methanol and acetone in this order
8. customby drying