Dimethylformamide → HCID 86761427, compound

HRID 1662927Notes

Reaction equation

HRID 1662927 reaction equation
COC1C=CC=C(C=1)C([O:21][C:22]([C:37]1[CH:42]=[CH:41]C=[CH:39][CH:38]=1)([C:29]1[CH:34]=[C:33](OC)[CH:32]=[CH:31][CH:30]=1)C1C=CC=CC=1)(C1C=CC=CC=1)C1C=CC=CC=1.Cl[CH:45](Cl)C(O)=O.[CH3:50][N:51](C)[CH:52]=[O:53]>>[N:51]([C:50]1[CH:39]=[CH:38][C:37]([C:22]([C:29]2[CH:30]=[CH:31][CH:32]=[CH:33][C:34]=2[CH3:45])=[O:21])=[CH:42][CH:41]=1)=[C:52]=[O:53]

Participants

4

Reactant 3

HCID 6228DimethylformamideHCID 6597Dichloroacetic AcidHCID 66905470HCID 66905470, compound

Product 1

HCID 86761427HCID 86761427, compound

Procedure

Modification of 5′-amino DNA: DNA to serve as the 3′-probe, carrying a 5′-deoxy-5′-amino nucleoside, is prepared by standard solid phase synthesis according to standard protocols. Phosphoramidites, including the 5′-amino nucleoside analog where the 5′-amino group is protected as a 5′-monomethoxytrityl ether, are available from Glen Research. The final detritylation will be done with 2% dichloroacetic acid for 15 min. The product is then treated with the isocyanato derivative in dimethylformamide at 25° C. The products are then purified by standard oligonucleotide HPLC (reversed phase C-18, water-acetonitrile eluant).

Workup

  1. customis prepared by standard solid phase synthesis
  2. customThe products are then purified by standard oligonucleotide HPLC (reversed phase C-18, water-acetonitrile eluant)

Sources & evidence

ORD record
ord-1f407eb6f8d14ebea600cf271c2dd7d9
Source dataset
uspto-grants-2011_10
Patent number
US08034563B1

Reaction notes

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