D-(+)-Cellobiose (CNP0226604.15) → D-(+)-Cellobiose (CNP0226604.15)

Participants

Reaction Conditions

Reaction Steps

Cellobiase activity: The adequately diluted enzyme solution and 1 mL of 15 mM cellobiose solution in citrate buffer (pH 4.8, 0.05 M) were mixed in equal volumes, followed by enzyme reaction at 50° C. for 30 minutes. Then, the enzyme reaction was terminated by heating at 100° C. for 5 minutes. The glucose concentration in the solution was measured using a laboratory test kit (Glu-CII, Wako Pure Chemicals, Tokyo, Japan). Herein, the enzyme amount at which reducing sugar is produced in an amount corresponding to 2 μmol of glucose or 1 μmol of cellobiose per minute was defined as 1 unit (U).

Provenance & Evidence