D-(+)-Cellobiose (CNP0226604.15) → D-(+)-Cellobiose (CNP0226604.15)

HRID 1662936Notes

Reaction equation

HRID 1662936 reaction equation
[CH2:1]([OH:23])[C@H:2]1[O:7][C@@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@H:12]([OH:17])[O:11][C@@H:10]2[CH2:18][OH:19])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22]>C([O-])(=O)CC(CC([O-])=O)(C([O-])=O)O>[O:8]=[CH:6][C@@H:5]([C@H:4]([C@@H:3]([C@@H:2]([CH2:1][OH:23])[OH:7])[OH:22])[OH:21])[OH:20].[CH2:1]([OH:23])[C@H:2]1[O:7][C@@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@H:12]([OH:17])[O:11][C@@H:10]2[CH2:18][OH:19])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22]

Participants

4

Reactant 1

HCID 10712D-(+)-Cellobiose (CNP0226604.15)

Product 2

HCID 10712D-(+)-Cellobiose (CNP0226604.15)HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Reagents, catalysts & solvents 1

HCID 31348CitrateSolvent

Conditions

Temperature
100 °C

Procedure

Cellobiase activity: The adequately diluted enzyme solution and 1 mL of 15 mM cellobiose solution in citrate buffer (pH 4.8, 0.05 M) were mixed in equal volumes, followed by enzyme reaction at 50° C. for 30 minutes. Then, the enzyme reaction was terminated by heating at 100° C. for 5 minutes. The glucose concentration in the solution was measured using a laboratory test kit (Glu-CII, Wako Pure Chemicals, Tokyo, Japan). Herein, the enzyme amount at which reducing sugar is produced in an amount corresponding to 2 μmol of glucose or 1 μmol of cellobiose per minute was defined as 1 unit (U).

Workup

  1. customfollowed by enzyme reaction at 50° C. for 30 minutes
  2. customThen, the enzyme reaction
  3. customwas terminated
  4. concentrationThe glucose concentration in the solution

Sources & evidence

ORD record
ord-6cfd5854bb104fbf9fb17c63f27ece7d
Source dataset
uspto-grants-2011_10
Patent number
US08034596B2

Reaction notes

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