反应详情

N-(2-aminoethyl)-3-{1-(4-sulfamoylphenyl)-5-p-tolyl-1H-pyrazol-3-yl}propanamide hydrochloride was reacted with N,N-diisopropylethylamine (DIPEA) in dichloromethane for 5 minutes at room temperature to produce N-(2-aminoethyl)-3-{1-(4-sulfamoylphenyl)-5-p-tolyl-1H-pyrazol-3-yl}propanamide which was then reacted with 5-(N-(14-carboxy-3,6,9,12-tetraoxatetradecyl)sulfamoyl)-2-(6-(diethylamino)-3-(diethyliminio)-3H-xanthen-9-yl)benzenesulfonate (produced as disclosed hereinabove with respect to Compound 27iii) in the presence of 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide hydrochloride (EDCl), 1-hydroxybenzotriazole (HOBt), and N,N-diisopropylethylamine (DIPEA) in dichloromethane at room temperature to produce Compound 30f as a dark red solid (57% yield).