反应详情

温度

120 °C

A solution of ethyl 4-chloro-1,6-diethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate (14.8 g, 52.6 mmol, e.g. which can be as prepared in Intermediate 3, first or alternative preparation thereof) in N-methyl-2-pyrrolidinone (140 ml) was treated with N,N-diisopropylethylamine (22.9 ml, 131 mmol) followed by 1,1-dimethylethyl 4-amino-1-piperidinecarboxylate (11.6 g, 57.9 mmol, commercially available e.g. from AstaTech) and the mixture was heated at 120° C. for 24 hours. The reaction mixture was cooled and poured into aqueous lithium chloride solution (5% LiCl, 1 L) and the aqueous phase was extracted with ethyl acetate (3×250 ml). The combined organic extracts were dried (Na2SO4) and the solvent was evaporated. The residue, an oil, was purified by column chromatography using a column of silica gel (ART9385, 500 ml) made up in 4:1 hexane:ethyl acetate, eluting with 4:1 hexane:ethyl acetate (500 ml) and then 2:1 hexane:ethyl acetate until the product eluted. The fractions containing the product were pooled and the solvent was evaporated to give the title compound (18.55 g) as an oil which solidified on standing. LCMS m/z 446 [MH+]; TRET=3.69 min.

1. customfirst or alternative preparation
2. temperatureThe reaction mixture was cooled
3. extractionthe aqueous phase was extracted with ethyl acetate (3×250 ml)
4. dry with materialThe combined organic extracts were dried (Na2SO4)
5. customthe solvent was evaporated
6. customThe residue, an oil, was purified by column chromatography
7. washethyl acetate, eluting with 4:1 hexane
8. washethyl acetate (500 ml) and then 2:1 hexane:ethyl acetate until the product eluted
9. additionThe fractions containing the product
10. customthe solvent was evaporated