HRID1678040

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1

HCID5886

Adenosine 5′-(trihydrogen diphosphate), 2′-(dihydrogen phosphate), P′→5′-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium

C21H29N7O17P3+

HCID14798

Sodium Hydroxide

HNaO

结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO

HCID107526

未命名化合物

HCID168006

Ethanone, 2-chloro-1-(3-chlorophenyl)-

C8H6Cl2O

PRODUCTS

生成物

HCID10375278

(R)-2-Chloro-1-(3-chlorophenyl)ethanol

C8H8Cl2O

收率 90.5%

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID1140

Toluene

C7H8

CONDITIONS

反应条件

温度: 30 °C

PROCEDURE

实验过程

To 50 ml of a culture solution of E. coli HB101 (pNTCMG1) prepared in the same manner as in Example 6, 5 g of glucose, 6 mg of NADP, 4 g of 2-chloro-1-(3′-chlorophenyl)ethanone and 4 g of toluene were added. The mixture was stirred at 30° C. for 24 hours while adjusting the pH of the mixture to 6.5 by adding 5 M sodium hydroxide dropwise. After completion of the reaction, the reaction solution was extracted with toluene. The solvent was removed and then an extracted material was analyzed. As a result, (R)-2-chloro-1-(3′-chlorophenyl)ethanol having an optical purity of 82.0% e.e. was obtained in a yield of 90.5%.

WORKUP

后处理

additionwere added
customAfter completion of the reaction
extractionthe reaction solution was extracted with toluene
customThe solvent was removed