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HRID1678042

反应详情

EQUATION

反应方程式

HRID 1678042 的结构方程式

REACTANTS

反应物

4
结构：NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
HCID5886

Adenosine 5′-(trihydrogen diphosphate), 2′-(dihydrogen phosphate), P′→5′-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium

C21H29N7O17P3+

结构：[Na+].[OH-]
HCID14798

Sodium Hydroxide

HNaO

结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
HCID107526

未命名化合物

结构：CC(C)(C)OC(=O)CC(=O)C[C@H](O)COC(=O)c1ccccc1
HCID15099849

未命名化合物

PRODUCTS

生成物

1
结构：CC(C)(C)OC(=O)C[C@H](O)C[C@@H](O)COCc1ccccc1
HCID11012348

未命名化合物

收率 92.3%

AUXILIARIES

试剂、催化剂与溶剂

1
结构：Cc1ccccc1
溶剂HCID1140

Toluene

C7H8

CONDITIONS

反应条件

温度
30 °C

PROCEDURE

实验过程

To 40 ml of a culture solution of E. coli HB101 (pNTCMG1) prepared in the same manner as in Example 6, 3 g of glucose, 6 mg of NADP, 4 g of tert-butyl (S)-6-benzoyloxy-5-hydroxy-3-oxohexanoate and 1.6 g of toluene were added. The reaction solution was stirred at 30° C. for 24 hours while adjusting the pH of the reaction solution to pH 6.5 by adding 5 M sodium hydroxide dropwise. After completion of the reaction, the reaction solution was extracted with toluene. The solvent was removed and then an extracted material was analyzed. As a result, tert-butyl (3R,5R)-6-benzyloxy-3,5-dihydroxyhexanoate having an excess rate of a diastereomer of 99.7% d.e. was obtained in a yield of 92.3%.

WORKUP

后处理

  1. additionwere added
  2. customAfter completion of the reaction
  3. extractionthe reaction solution was extracted with toluene
  4. customThe solvent was removed