Phosphate → D-(-)-Fructose

HRID 1679088Notes

Reaction equation

[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].[O-]S([O-])(=O)=O.[Mg+2].P([O-])([O-])([O-])=O>O>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

Conditions

Temperature: 70 °C

Procedure

A mixture of 0.2 ml of 1 M aqueous D-glucose solution, 0.2 ml of 0.05 M aqueous MgSO4 ·7H2O solution, 0.2 ml of 0.5 M aqueous phosphate buffer (pH = 7.2) and a given amount of aqueous solution of extracted glucose isomerase is diluted with water to make it to 2 ml. The mixture is maintained at a temperature of 70° C for 60 minutes to effect the conversion which is terminated by the addition of 2 ml of 0.5 M perchloric acid. The amount of fructose produced is determined by the cystein carbazole method. The value obtained by dividing the amount of fructose produced by the amount of the extracted glucose isomerase solution is the activity, the unit of which is expressed by the abbreviation "U".

Workup

customis terminated by the addition of 2 ml of 0.5 M perchloric acid

Sources & evidence

ORD record: ord-361f0de2702b42babce8ebd09c96a7a7
Source dataset: uspto-grants-1978_03
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04078970

Reaction notes

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