D-(-)-Fructose → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 1679089Notes

Reaction equation

HRID 1679089 reaction equation
C1C2NC3C(=CC=CC=3)C=2C=CC=1.N[C@H](C(O)=O)CS.[OH:21][CH2:22][C:23]([C@H:25]([C@@H:27]([C@@H:29]([CH2:31][OH:32])[OH:30])[OH:28])[OH:26])=[O:24]>>[O:21]=[CH:22][C@@H:23]([C@H:25]([C@@H:27]([C@@H:29]([CH2:31][OH:32])[OH:30])[OH:28])[OH:26])[OH:24]

Participants

3

Reactant 2

HCID 5984D-(-)-FructoseHCID 22825735HCID 22825735, compound

Product 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Procedure

A mixture of 0.2 ml of 1 M aqueous D-glucose solution, 0.2 ml of 0.05 M aqueous MgSO4 ·7H2O solution, 0.2 ml of 0.5 M aqueous phosphate buffer (pH = 7.2) and a given amount of aqueous solution of extracted glucose isomerase is diluted with water to make it to 2 ml. The mixture is maintained at a temperature of 70° C for 60 minutes to effect the conversion which is terminated by the addition of 2 ml of 0.5 M perchloric acid. The amount of fructose produced is determined by the cystein carbazole method. The value obtained by dividing the amount of fructose produced by the amount of the extracted glucose isomerase solution is the activity, the unit of which is expressed by the abbreviation "U".

Workup

  1. customThe value obtained

Sources & evidence

ORD record
ord-e2063f526f4240dbaa99142044698f4d
Source dataset
uspto-grants-1978_03
Patent number
US04078970

Reaction notes

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