Phosphate → D-(-)-Fructose

HRID 1679090Notes

Reaction equation

HRID 1679090 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].[O-]S([O-])(=O)=O.[Mg+2].P([O-])([O-])([O-])=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

4

Reactant 3

HCID 1061PhosphateHCID 24083Sulfuric acid magnesium salt (1:1)HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×

Product 1

HCID 5984D-(-)-Fructose

Conditions

Temperature
70 °C

Procedure

To one liter of an aqueous solution containing glucose, MgSO4 ·7H2O and phosphate buffer at a concentration of 0.1 M, 0.005 M and 0.05 M, respectively, is added a given amount of an insolubilized glucose isomerase. The mixture thus obtained is slowly stirred at a temperature of 70° C for 60 minutes to effect the isomerization. Then, the insolubilized isomerase is separated from the reacted solution and the amount of fructose so produced is determined according to the cystein carbazol method. The activity is calculated as in 1 above.

Workup

  1. customThe mixture thus obtained
  2. customThen, the insolubilized isomerase is separated from the reacted solution

Sources & evidence

ORD record
ord-2cdffff6a07645918d046545bdeb076b
Source dataset
uspto-grants-1978_03
Patent number
US04078970

Reaction notes

Loading…